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      mg
      mL

      Example — change to your numbers.

      Draw to10 units
      0.1 mL

      Per unit
      25 mcg
      Strength
      2.5 mg/mL
      Doses per vial
      20

      Math only — double-check against your vial label.

      How to mix a vial

      1. Wipe both tops

        Wash your hands, then swab the rubber tops of the peptide vial and the water vial with alcohol. Let them dry.

      2. Draw the water

        Use bacteriostatic (BAC) water — sterile water with a preservative, sold for mixing peptides. With a new sterile syringe, draw the amount you entered in the calculator. A 1 mL syringe filled twice is 2 mL.

      3. Run it down the wall

        Push the needle into the peptide vial at an angle and let the liquid trickle down the glass, not onto the powder.

      4. Swirl, don’t shake

        Gently swirl or roll the vial until the liquid is completely clear. Give it a few minutes if needed.

      5. Label it and keep it cold

        Write the date and what you mixed (for example 5 mg + 2 mL) on the vial. Keep it in the fridge at 2–8°C (35.6–46.4°F), away from light.

      This is general practice. If your product came with its own diluent or instructions, follow those. Not sure what to buy? See the supplies checklist.

      How to read a U-100 syringe

      Close-up of an insulin syringe drawn to 10 unitsEach small line is 1 unit, which is 0.01 mL. Ten units is 0.10 mL. Read the line at the top edge of the plunger's rubber tip, the edge nearest the needle.05101 unit = 0.01 mLRead here10 units = 0.10 mL
      Pull the plunger back until the top edge of its rubber tip — the edge nearest the needle — sits on your line.

      Use the smallest syringe that fits your draw

      • 30units0.3 mL syringeLines every 1 unit (some show ½). Best for draws under 30 units.
      • 50units0.5 mL syringeLines every 1 unit. For draws of 30–50 units.
      • 100units1 mL syringeLines every 2 units — count carefully. For draws over 50 units.
      The math behind it

      Strength = amount in the vial ÷ mL of water.
      Units to draw = your dose ÷ strength × 100.

      Example: 5 mg is 5,000 mcg. In 2 mL that is 2,500 mcg per mL. A 250 mcg dose is 250 ÷ 2,500 × 100 = 10 units (0.10 mL).

      Advanced: plan a water volume

      For pens & cartridges, or a round number per unit

      Using a V2 pen? 1 click = 1 unit = 0.01 mL. Enter the vial and what the cartridge holds, and this suggests a water volume with a simple amount per unit. What a pen setup needs

      mg

      Total amount printed on the vial.

      mL

      Usable capacity of the vial or pen cartridge you are filling.

      1 dial unit = 0.01 mL on the documented V2 model. Confirm your pen’s scale.

      Vial sizes & blend contents Optional

      Listed amounts are packaging examples. Check your actual label.

      Pens & other measuring tools

      Device units measure volume. The strength of your mix decides how much peptide is in each one.

      V2 peptide pen1 click = 0.01 mLDocumented 1-unit V2 models
      One dial click
      1 unit
      Cartridge holds
      3 mL
      Most per dose
      60 units · 0.60 mL

      Read the numbered dose window. Whole units only; check the spec for your exact model.

      Other measuring toolsUse the mL scalemL syringes and calibrated pipettes
      0.01 mL
      10 µL
      0.10 mL
      100 µL
      Amount
      mL × strength

      Drops, sprays and other pens need their own verified volume per click.

      3 mL is what a cartridge holds, not how much you must mix. The original vial and the pen cartridge are different containers.

      Device references
      AVERON V2 specifications ↗SilverLeaf V2 unit reference ↗FDA: syringe unit conversions ↗Specifications checked October 12, 2026.